5/18/2023 0 Comments Seed box model 3dThese treatments for the sample preparation often affect the native structure of organisms, thereby making the interpretation of results difficult. Originally the plant tissues were observed using a light microscopy after a series of treatments comprising the fixing, the sectioning, and the staining. The present method may provide a unique way to observe live plant tissues at micron resolutions without structural perturbations due to the sample preparation.įinding in plant biology depends highly upon the imaging technique. Our results confirmed an availability of the laboratory-based X-ray phase-contrast CT for a 3D-structural study on the development of small seeds. The manual segmentation allowed a 3D rendering of embryos at three different stages in the germination, which visualized an overall morphological change of the embryo upon germination as well as a spatial arrangement of cells inside the embryo. The phase-retrieved tomograms from both the dry and the wet seeds revealed a cellular level of spatial resolutions that were enough to resolve cells in the seeds, and provided enough contrasts to delineate the boundary of embryos manually. In addition to a dry seed, wet seeds on germination with the poorer contrasts were tried. The phase-retrieval technique was applied to enhance contrasts in the projection images. ResultsĪ live pansy seed within 2 mm in size was simply mounted inside a plastic tube and irradiated by in-house X-rays to collect projection images using a laboratory-based X-ray microscope. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique.
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